MIQE指南

PCR论文发表最低标准

参考文献

Bustin SA et al. (2009). The MIQE guidelines: Minimum information for

publication of quantitative real-time PCR experiments. Clin Chem 55, 611-622.Denisov V et al. (2008). Development and validation of RQI: An RNA quality indicator for the Experion automated electrophoresis system. Bio-Rad Bulletin 5761

Fleige S and Pfaffl MW (2006). RNA integrity and the effect on the real-time qRT-PCR performance. Mol Aspects Med 27, 126-139.

Garson JA et al. (2009). Unreliable real-time PCR analysis of human endogenous retrovirus-w (HERV-W) RNA expression and DNA copy number in multiple sclerosis. AIDS Res Hum Retroviruses 25, 377-378; author reply 379-381.Gingrich J et al. (2006). Effect of RNA degradation on the data quality in quantitative PCR and microarray experiments. Bio-Rad Bulletin #5452 Gutierrez L et al. (2008). The lack of a systematic validation of reference genes: A serious pitfall undervalued in reverse transcription-polymerase chain reaction (RT-PCR) analysis in plants. Plant Biotechnol J 6, 609-618.

Hellemans J et al. (2007). Qbase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biology 8, R19.

Huggett J et al. (2005). Real-time RT-PCR normalization; strategies and considerations. Genes Immun 6, 279-284.

Imbeaud S et al. (2005). Towards standardization of RNA quality assessment using user-independent classifiers of microcapillary electrophoresis traces. Nucleic Acids Res 33, e56.

Lefever S et al. (2009). RDML: Structured language and reporting guidelines for real-time quantitative PCR data. Nucleic Acids Res Advance Access published February 17, 2009 (accessed May 21, 2009).

Pfaffl, M. (2001). A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29, e45.

Rozen S and Skaletsky HJ (2000). Primer3 on the www for general users and for biologist programmers. In Bioinformatics methods and protocols: Methods in molecular biology, ed. (Totowa, NJ: Humana Press), pp. 365-386.

Vandesompele J et al. (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3, research0034.1-0034.11

Zuker M (2003). MFOLD web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31, 3406-3415.

Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s United States Patent 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary

diagnostics. This product is covered by one or more of the following U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent Nos. 6,767,512 and 7,074,367.

EvaGreen is a trademark of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc. to sell reagents containing EvaGreen dye for use in real-time PCR, for research purposes only.

Practice of the patented 5' Nuclease Process requires a license from Applied Biosystems. The purchase of these products includes an

immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser’s own internal research when used with the separate purchase of Licensed Probe. No other patent rights are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

CFX Manager software is for use with authorized real-time instrument or thermal cycler. Please see user manual for complete licensing statement.Nanodrop is a trademark of Thermo Fisher Scientific, Inc.

Information in this tech note was current as of the date of writing (2009) and not necessarily the date this version (rev A, 2009) was published.

Acknowledgements

We are grateful to Dr Jo Vandesompele for his insightful comments and helpful contributions to this paper.

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